HYPERTHERMIA AND HOST-CELL REACTIVATION OF ADENOVIRUS 12
- 1 March 1978
- journal article
- research article
- Published by Canadian Science Publishing in Canadian Journal of Genetics and Cytology
- Vol. 20 (1) , 35-40
- https://doi.org/10.1139/g78-006
Abstract
Exposure of cultured human fibroblasts to hyperthermia delayed the host-cell reactivation of UV-irradiated human adenovirus type 12 (AD12). The experimental design consisted of irradiating human AD12 with UV doses ranging from 180 to 1800 ergs/mm2, infecting human cell populations at 37 °C, exposing the infected cells for 7 h to 39.5 °C and 41.8 °C, returning them to 37 °C and estimating the frequency of cells with intranuclear viral inclusion bodies (IB) 41 and 89 h after hyperthermia treatment. Hyperthermia reduced the fractions of fibroblasts with viral IB in the 41 h samples. By 89 h the capacity to form IB in the treated cells was comparable to that in control cells. Hyperthermia of 39.5 and 41.8 °C for 7 h did not affect the replication of nonirradiated AD12. The pattern of host-cell reactivation of AD12 following hyperthermia was compared to that in DNA repair deficient xeroderma pigmentosum cell populations.This publication has 15 references indexed in Scilit:
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