A Mammalian Histone Deacetylase Related to the Yeast Transcriptional Regulator Rpd3p
- 19 April 1996
- journal article
- research article
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 272 (5260) , 408-411
- https://doi.org/10.1126/science.272.5260.408
Abstract
Trapoxin is a microbially derived cyclotetrapeptide that inhibits histone deacetylation in vivo and causes mammalian cells to arrest in the cell cycle. A trapoxin affinity matrix was used to isolate two nuclear proteins that copurified with histone deacetylase activity. Both proteins were identified by peptide microsequencing, and a complementary DNA encoding the histone deacetylase catalytic subunit (HD1) was cloned from a human Jurkat T cell library. As the predicted protein is very similar to the yeast transcriptional regulator Rpd3p, these results support a role for histone deacetylase as a key regulator of eukaryotic transcription.Keywords
This publication has 17 references indexed in Scilit:
- Identification of a Gene Encoding a Yeast Histone H4 AcetyltransferasePublished by Elsevier ,1995
- Trichostatin A and trapoxin: Novel chemical probes for the role of histone acetylation in chromatin structure and functionBioEssays, 1995
- The ancient regulatory-protein family of WD-repeat proteinsNature, 1994
- Decoding the nucleosomeCell, 1993
- A retinoblastoma-binding protein related to a negative regulator of Ras in yeastNature, 1993
- Transcriptional silencing in yeast is associated with reduced nucleosome acetylation.Genes & Development, 1993
- Identification of calcineurin as a key signalling enzyme in T-lymphocyte activationNature, 1992
- Modifiers of position effect are shared between telomeric and silent mating-type loci in S. cerevisiaeCell, 1991
- Genetic Analysis of Histone H4: Essential Role of Lysines Subject to Reversible AcetylationScience, 1990
- Both positive and negative regulators of HO transcription are required for mother-cell-specific mating-type switching in yeastCell, 1987