Packed Column Supercritical Fluid Chromatography/Mass Spectrometry for High-Throughput Analysis. Part 2
- 26 August 1999
- journal article
- research article
- Published by American Chemical Society (ACS) in Analytical Chemistry
- Vol. 71 (19) , 4223-4231
- https://doi.org/10.1021/ac9902906
Abstract
A supercritical fluid chromatograph was previously interfaced to a mass spectrometer (SFC/MS) and the system evaluated for applications requiring high sample throughput using negative-mode atmospheric-pressure chemical ionization (APCI) (Ventura et al. Anal.Chem.1999, 71, 2410−2416). This report extends the previous work demonstrating the effectiveness of SFC/MS, using positive ion APCI for the analysis of compounds with a wide range of polarities. Substituting SFC/MS for LC/MS results in substantial time saving, increased chromatographic efficiency, and more precise quantitation of sample mixtures. Flow injection analysis (FIA) also benefits from our SFC/MS system. A broader range of solvents is compatible with the SFC mobile phase compared with LC/MS, and solutes elute more rapidly from the SFC/MS system, reducing sample carryover and cycle time. Our instrumental setup also allows for facile conversion between LC/MS and SFC/MS modes of operation.Keywords
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