Rapid Detection ofAgrobacterium tumefaciensin Grapevine Propagating Material and the Basis for an Efficient Indexing System
- 1 January 1986
- journal article
- research article
- Published by Scientific Societies in Plant Disease
- Vol. 70 (6) , 566-568
- https://doi.org/10.1094/pd-70-566
Abstract
Sap from hardwood stem pieces of five grape cultivars and 10 rootstock selections was collected by a water-displacement procedure and plated on a medium selective for the Agrobacterium tumefaciens biovar 3. Of 36 strains of A. tumefaciens recovered, 86% were identified as biovar 3 according to their physiological and virulence characteristics; 90% of the apical portions of grape canes sampled were free of A. tumefaciens. Our data provide the basis for a simple, yet rapid indexing system for the detection of low populations of unevenly distributed A. tumefaciens cells in grape wood. With this procedure, it should be possible to obtain A. tumefaciens-free propagating material.This publication has 0 references indexed in Scilit: