Iron-regulated proteins inPhanerochaete chrysosporium andLentinula edodes: Differential analysis by sodium dodecyl sulfate polyacrylamide gel electrophoresis and two-dimensional polyacrylamide gel electrophoresis profiles

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Abstract
Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS‐PAGE) and two‐dimensional gel electrophoresis (2‐DE) were used to identify iron‐responsive proteins in the white‐rot species (Phanerochaete chrysosporium and Lentinula edodes), by comparing the differential patterns of cellular and membrane proteins obtained from iron‐sufficient and iron‐deficient mycelia. Six cellular proteins induced by iron restriction have been observed in SDS‐PAGE for P. chrysosporium and twelve for L. edodes. In 2‐DE, the numbers of iron‐restricted induced proteins were 12 and 9, respectively, in a resolution range of 15–60 kDa and pI 4.5–8.1. SDS‐PAGE for the plasma membrane protein did not show differences, whereas the outer‐membrane protein profile showed 6 and 5 proteins induced by iron depletion in P. chrysosporium and L. edodes, respectively. The results presented here are important data to unravel mechanisms of biosynthesis and/or transport of the iron‐complexing agents in ligninolytic fungi and to further correlate them to the ligninolytic processes.