The 3' end formation in small RNAs.
- 1 January 2002
- journal article
- review article
- Vol. 10, 59-78
Abstract
Small RNAs are a major class of RNAs along with transfer RNAs, ribosomal RNAs, and messenger RNAs. They vary in size from less than 100 nucleotides to several thousand nucleotides and have been identified and characterized both in prokaryotes and eukaryotes. Small RNAs participate in a variety of cellular functions including regulating RNA synthesis, RNA processing, guiding modifications in RNA, and in transport of proteins. Small RNAs are generated by a series of posttranscriptional processing steps following transcription. While RNA 5' end structure, 5' cap formation, and RNA processing mechanisms have been fairly well characterized, the 3' end processing is poorly understood. Recent data point to an emerging theme in small RNAs metabolism in which the 3' end processing is mediated by the exosome, a large multienzyme complex. In addition to removal of nucleotides by the exosome, there is simultaneous rebuilding of the 3' end of some small RNA by adenylation and/or uridylation. This review presents a picture of both degradative and rebuilding reactions operative on the 3' end of some small RNA molecules in prokaryotes and eukaryotes.Keywords
This publication has 99 references indexed in Scilit:
- Formation of 2′,3′-Cyclic Phosphates at the 3′ End of Human U6 Small Nuclear RNA in VitroJournal of Biological Chemistry, 1997
- Cloning and Characterization of the ArabidopsisCyclic Phosphodiesterase Which Hydrolyzes ADP-ribose 1“,2”-Cyclic Phosphate and Nucleoside 2′,3′-Cyclic PhosphatesPublished by Elsevier ,1997
- Maturation Pathways for E. coli tRNA Precursors: A Random Multienzyme Process In VivoCell, 1996
- A mammalian gene with introns instead of exons generating stable RNA productsNature, 1996
- Processing of fibrillarin-associated snoRNAs from pre-mRNA introns: An exonucleolytic process exclusively directed by the common stem-box terminal structureBiochimie, 1996
- Alteration of the RNA polymerase specificity of U3 snRNA genes during evolution and in vitroCell, 1991
- Formation of the 3′ end of U1 snRNA requires compatible snRNA promoter elementsCell, 1986
- Sequences required for 3′ end formation of human U2 small nuclear RNACell, 1985
- Ribonucleoprotein organization of eukaryotic RNAJournal of Molecular Biology, 1985
- 7S RNA, containing 5S ribosomal RNA and the termination stem, is a specific substrate for the two RNA processing enzymes RNase III and RNase EBiochemistry, 1984