Genetic reconstruction of a functional transcriptional regulatory network
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- 8 April 2007
- journal article
- research article
- Published by Springer Nature in Nature Genetics
- Vol. 39 (5) , 683-687
- https://doi.org/10.1038/ng2012
Abstract
Although global analyses of transcription factor binding provide one view of potential transcriptional regulatory networks1,2, regulation also occurs at levels distinct from transcription factor binding3,4. Here, we use a genetic approach to identify targets of transcription factors in yeast and reconstruct a functional regulatory network. First, we profiled transcriptional responses in S. cerevisiae strains with individual deletions of 263 transcription factors. Then we used directed-weighted graph modeling and regulatory epistasis analysis to identify indirect regulatory relationships between these transcription factors, and from this we reconstructed a functional transcriptional regulatory network. The enrichment of promoter motifs and Gene Ontology annotations provide insight into the biological functions of the transcription factors.Keywords
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