A STUDY OF THE KINETICS OF CHLORPROMAZINE SULFOXIDE BY A SPECIFIC RADIOIMMUNOASSAY AFTER A SINGLE ORAL DOSE OF CHLORPROMAZINE IN HEALTHY-VOLUNTEERS

  • 1 January 1983
    • journal article
    • research article
    • Vol. 226  (3) , 833-838
Abstract
Antibody specific for chlorpromazine sulfoxide (CPZSO) was produced in rabbits immunized with a hapten-bovine serum albumin conjugate, which was prepared by linking the 10-alkyl side chain of CPZSO to the protein molecule via a 2-C bridge. A simple radioimmunoassay was developed which can measure < 20 pg of CPZSO in plasma. The assay has adequate specificity so that isolation of CPZSO is unnecessary. It was used together with a previously developed chlorpromazine (CPZ) radioimmunoassay to determine the concentrations of CPZ and CPZSO in plasma samples from 5 healthy volunteers after they had received a single 50-mg oral dose of CPZ. Evidently, a significant portion of CPZ is metabolized in CPZSO during presystemic absorption. There are differences to findings previously reported in the plasma concentration ratios of CPZSO to CPZ. The possible reasons for these differences are discussed.