OBSERVATIONS ON SOME OF THE FACTORS INVOLVED IN FILTRATION EXPERIMENTS

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Abstract
Neither the rate of flow of distilled water nor the pressure required to bubble air through a filter under water is a safe guide as to whether or not the filter will hold back bacteria. An old culture of Bacillus prodigiosus will pass through a filter which is impermeable to a young culture. The nature of the suspension fluid is of great importance in determining whether or not particles of a given size will pass through a filter. Victoria blue B will pass through Berkefeld filters, if dissolved in hormone broth. Young cultures of B. prodigiosus will pass through Berkefeld filters V and N, if suspended in a modified hormone broth or in alkaline extracts of certain animal or vegetable cells. The chemical composition of the substance present in hormone broth and in certain cell extracts which facilitates filtration through Berkefeld filters has not been determined.

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