INTRINSIC BIREFRINGENCE OF GLYCERINATED MYOFIBRILS
Open Access
- 1 December 1971
- journal article
- Published by Rockefeller University Press in The Journal of cell biology
- Vol. 51 (3) , 763-771
- https://doi.org/10.1083/jcb.51.3.763
Abstract
Patterns of intrinsic birefringence were revealed in formalin-fixed, glycerinated myofibrils from rabbit striated muscle, by perfusing them with solvents of refractive index near to that of protein, about 1.570. The patterns differ substantially from those obtained in physiological salt solutions, due to the elimination of edge- and form birefringence. Analysis of myofibrils at various stages of shortening has produced results fully consistent with the sliding filament theory of contraction. On a weight basis, the intrinsic birefringence of thick-filament protein is about 2.4 times that of thin-filament protein. Nonadditivity of thick- and thin-filament birefringence in the overlap regions of A bands may indicate an alteration of macromolecular structure due to interaction between the two types of filaments.Keywords
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