STUDIES ON IMMUNE HEMOLYSIS: PREPARATION OF A STABLE AND HIGHLY REACTIVE COMPLEX OF SENSITIZED ERYTHROCYTES AND THE FIRST COMPONENT OF COMPLEMENT (EAC'1); INACTIVATION OF CELL-FIXED C'1 BY SOME COMPLEMENT REAGENTS

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Abstract
The interaction of sensitized sheep erythrocytes with guinea pig serum partially inactivated by treatment with hydrazine or ether was studied. In both cases the complex so formed (EAC''I) was hemolytically reactive with a complement (C'') reagent lacking in the first component of C (Rl); but this complex was not reactive with a C'' reagent lacking in any other component. This hemolytic reactivity disappeared rapidly during exposure of the complex to hydrazine-treated or decomplemented guinea pig serum. The hemolytic reactivity persisted, however, during exposure of the complex to ether-treated complement. The capacity of hydrazine-treated and decomplemented guinea pig serum to inactivate the complex is markedly reduced by treating these reagents with ether. It is concluded that there is an ether-labile factor in guinea pig serum, in hydrazine-treated guinea pig serum, and in decomplemented guinea pig serum that inactivates the first component of C. A highly reactive and stable EAC''l complex may be formed by exposing sensitized erythrocytes to ether-treated C'' in the cold.