Identification and characterization of a lower plant Ypt/Rab guanosine dissociation inhibitor (GDI)
- 4 November 1996
- journal article
- Published by Wiley in FEBS Letters
- Vol. 396 (2-3) , 298-304
- https://doi.org/10.1016/0014-5793(96)01068-x
Abstract
The cDNA encoding a Ypt/Rab guanosine dissociation inhibitor (Ypt-GDI) was isolated from the multicellular green alga Volvox carteri, representing the first complete plant gdi gene described. The gdiV1 gene occurs as a single copy in the algal genome, indicating that its product regulates all YptV proteins from Volvox. The derived GDI protein (GDIV1p) shows high similarity to animal and fungal GDIs. A specific antibody developed against GDIV1p detected the protein throughout the whole Volvox life-cycle. GDIV1p was localized in the cytoplasm and in the algal flagellum. This is in line with earlier findings of a dual localization of Ypt proteins both in the cell body and in the motility organelle, and indicates a novel role of the GDI/Ypt system, possibly in intraflagellar transport.Keywords
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