Somatostatin Inhibition of Intrinsic Factor Secretion from Isolated Guinea Pig Gastric Glands

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Abstract
The aim of our study was to examine the direct effect of somatostatin on histamine- and pentagastrin-stimulated intrinsic factor (IF) release in collagenase-dispersed guinea pig gastric glands. The effect of somatostatin (1011 M to 10-6 M) on half-maximal doses of histamine (10-6 M), pentagastrin (10-6 M), and both histamine and pentagastrin together was tested. All tested concentrations of histamine significantly stimulated IF release. Pentagastrin (10-10 M to 10-6 M) inconsistently stimulated IF release. The quantity of IF release stimulated by histamine and pentagastrin together was approximately the additive sum of that produced by either agent alone. Somatostatin (10-6 M) inhibited histamine-stimulated (10-6 M) IF release by 69.9 .+-. 7.2% and the combination of histamine (10-6 M) and pentagastrin (10-6 M) by 64.2 .+-. 9.1%. This is the first in vitro demonstration that somatostatin inhibits IF release.