Method for the Determination or Rates of Spore Inactivation at Ultra-High Temperatures

Abstract
A method is described for determining the rate of spore inactivation in the temperature range of 119-130[degree]C in 3:1 concentrated whole milk and in 0.067 [image] phosphate buffer. A design for a device to measure the rate of heat penetration of inoculated medium by means of a heat penetration tube containing a thermistor probe and held in a special metal holder is given. Heat penetration factors (time in sec.) using high temperature and low temperature polyalklene glycol in the heating bath at a temperature of 119.2[degree]C were as follows: Lag factor (j) for concentrate, 1.59; for buffer, 1.29; Heating curve slope (f h) for concentrate, 22.0; for buffer, 21.2 in low temperature glycol. The values in high temperature glycol were for the concentrate; j = 1.97 and f h = 22.9; for the buffer, j = 1.73 and f h = 22.3.

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