Sex pheromone plasmid pAD1-encoded surface exclusion protein ofEnterococcus faecalis
- 1 May 1992
- journal article
- Published by Springer Nature in Molecular Genetics and Genomics
- Vol. 233 (1-2) , 161-168
- https://doi.org/10.1007/bf00587575
Abstract
During conjugative transfer of sex pheromone plasmids ofEnterococcus faecalis a so-called surface exclusion protein reduces the frequency with which these plasmids are transferred to cells already possessing the same plasmid. We report here the DNA sequence of a 3 .8 kb fragment of the sex pheromone plasmid pAD1 containing the structural genesea1 for surface exclusion protein and a small open reading frame (ORF) upstream ofsea1. Surface exclusion protein Seal was found to be highly homologous to the surface exclusion protein Sec10 encoded by the sex pheromone plasmid pCF10. Hybridization studies with DNA probes derived from the structural gene seal demonstrated that, with the exception of pAM373, all known sex pheromone plasmids carry a homologous gene. These studies also indicated that the genetic organization is similar in these plasmids, with the structural gene for surface exclusion protein being located 5′ to that for aggregation substance.Keywords
This publication has 23 references indexed in Scilit:
- Transcriptional control of sex‐pheromone‐inducible genes on plasmid pAD1 of Enterococcus faecalis and sequence analysis of a third structural gene for (pPD1 ‐encoded) aggregation substanceMolecular Microbiology, 1992
- Nucleotide sequence of the sex pheromone inhibitor (iAD1) determinant ofEnterococcus faecalis conjugative plasmid pAD1Plasmid, 1990
- Conservation of a hexapeptide sequence in the anchor region of surface proteins from Gram‐positive cocciMolecular Microbiology, 1990
- Amino acid sequence of pheromone‐inducible surface protein in Enterococcus faecalis, that is encoded on the conjugative plasmid pPD1FEBS Letters, 1990
- Sequence analysis of Enterococcus faecalis aggregation substance encoded by the sex pheromone plasmid pAD1Molecular Microbiology, 1990
- Genetic functions and cell–cell interactions in the pheromone‐inducible plasmid transfer system of Enterococcus faecalisMolecular Microbiology, 1990
- Sex pheromones and plasmid transfer in Enterococcus faecalisPlasmid, 1989
- Unidirectional digestion with exonuclease III creates targeted breakpoints for DNA sequencingGene, 1984
- Plasmid Content of Streptococcus faecalis Strain 39-5 and Identification of a Pheromone (cPD1)-induced Surface AntigenMicrobiology, 1983
- Plasmid transfer in Streptococcus faecalis: Production of multiple sex pheromones by recipientsPlasmid, 1979