An Electrophoretic Analysis of Schistosoma mansoni Soluble Egg Antigen Preparation

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Abstract
S. mansoni soluble egg antigen (SEA) was examined electrophoretically. Sodium dodecyl sulfate (SDS) electrophoresis of SEA revealed an extremely heterogeneous protein composition. At least 18-20 distinct bands stained with Coomassie blue and at least 6 bands stained with periodic acid Schiff (PAS). Four of the PAS-positive bands stained only faintly with Coomassie blue. The estimated MW range for these proteins was between 16,000 and 200,000 daltons. An acid soluble fraction was isolated from SEA which contained 5 of the 6 glycoproteins. An immunoelectrophoretic analysis of SEA revealed at least 5 distinct precipitin arcs when developed with serum from mice infected with S. mansoni for 16 wk.