Gibberellin-induced Changes in Protein Synthesis and Enzyme Activity in Shoot Apices ofTrifolium pratense

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Abstract
A mutant remaining vegetative in normally inductive long-day conditions was used. Application of exogenous gibberellin A3 (GA3) produced normal flowering under inductive long-days. Isolated apices from treated and control plants were studied with regard to changes in size and rate of protein synthesis, as measured by leucine incorporation. Two protein synthesis peaks were detected, one during the first 4 days after CA2 application and a second, larger peak with a maximum at day 15. No consistent increases in acid-phosphatase, aminotransferase, amylase, or peroxidase specific activities could be associated with these peaks but an almost complete repression of alanine aminotransferase occurred during the second protein synthesis period. The physiological implications are discussed.

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