Ribosome‐associated protein that inhibits translation at the aminoacyl‐tRNA binding stage
Open Access
- 1 May 2001
- journal article
- Published by Springer Nature in EMBO Reports
- Vol. 2 (5) , 399-402
- https://doi.org/10.1093/embo-reports/kve091
Abstract
We have recently isolated and characterized a novel protein associated with Escherichia coli ribosomes and named protein Y (pY). Here we show that the ribosomes from bacterial cells growing at a normal physiological temperature contain no pY, whereas a temperature downshift results in the appearance of the protein in ribosomes. The protein also appears in the ribosomes of those cells that reached the stationary phase of growth at a physiological temperature. Our experiments with cell‐free translation systems demonstrate that the protein inhibits translation at the elongation stage by blocking the binding of aminoacyl‐tRNA to the ribosomal A site. The function of the protein in adaptation of cells to environmental stress is discussed.Keywords
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