High voltage electron microscopic studies of endothelial cell tubular structures in the mouse blood-brain barrier following brain trauma
- 1 January 1989
- journal article
- research article
- Published by Springer Nature in Acta Neuropathologica
- Vol. 77 (5) , 480-488
- https://doi.org/10.1007/bf00687249
Abstract
High-voltage electron microscopy was applied to the study of endothelial cell (EC) transport of macromolecules in a murine model of blood-brain barrier injury to study the role of the EC canalicular system following brain insult. Semithick sections from mouse brains subjected to acute (2–3 h) mechanical trauma demonstrated permeation of intravenously injected horseradish peroxidase via tubular structures either (a) in the absence of lysosome-associated structures in close proximity, or (b) in association with lysosomes, dense bodies or multivesicular bodies. Our data suggest a dual-purposed system of tubules, one portion that supplies the metabolic requirements of the cell and another portion, suggested to be more limited, that opens up as a result of brain injury.This publication has 51 references indexed in Scilit:
- A low-viscosity epoxy resin embedding medium for electron microscopyPublished by Elsevier ,2004
- Capillary junctions of the rat are not affected by osmotic opening of the blood-brain barrierActa Neuropathologica, 1984
- In vivo endocytosis by bristle coated pits of protein tracers and their intracellular transport in the endothelial cells lining the sinuses of the liver: II. The endosomal-lysosomal transformationJournal of Ultrastructure Research, 1983
- In vivo endocytosis by bristle coated pits of protein tracers and their intracellular transport in the endothelial cells lining the sinuses of the liverJournal of Ultrastructure Research, 1983
- Increased transendothelial channel transport of cerebral capillary endothelium in stroke-prone SHR.Stroke, 1983
- Tannic acid tracer analysis of permeability pathways in the capillaries of the rete mirabile: demonstration of the discreteness of endothelial vesiclesJournal of Ultrastructure Research, 1982
- A Simple Screening Procedure for Evaluating Central Nervous System Tissue Sections Showing Structural and Cytochemical Alterations of the Blood-Brain BarrierStain Technology, 1981
- Three-dimensional organization of plasmalemmal vesicles in endothelial cells. An analysis by serial sectioning of frog mesenteric capillariesJournal of Ultrastructure Research, 1980
- The effect of hyperglycemia on brain capillary permeability in the lizard, Anolis carolinensis. A freeze-fracture analysis of blood-brain barrier pathologyBrain Research, 1979
- Phosphotungstic acid as a marker for the endocytic-lysosomal system (vacuolar apparatus) including transfer tubules of the lining cells of the sinusoids in the bone marrow and liverJournal of Ultrastructure Research, 1977