PREPARATION OF A STABLE NONEFFECTIVE SOLUBLE INFLUENZA A ANTIGEN

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Abstract
When soluble influenza antigen is treated with formaldehyde to render it noninfective, the excess formaldehyde present causes a large loss of antigenicity on lyophilization. It has been shown previously that neutralization of the formaldehyde with ammonium hydroxide would overcome this difficulty. In the present study, a wide range of chemical compounds has been investigated to seek other reagents effective in neutralizing the formaldehyde present in treated soluble influenza antigen and which would possibly have advantages over ammonium hydroxide. It has been found that urea, dibasic ammonium phosphate, and sodium bisulphite are as effective as ammonium hydroxide, and possess the added advantage of not requiring any pH adjustment in the process.

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