A new extraction of arginine vasopressin from blood: The use of octadecasilyl-silica
- 1 August 1980
- journal article
- research article
- Published by Springer Nature in Pflügers Archiv - European Journal of Physiology
- Vol. 387 (1) , 79-81
- https://doi.org/10.1007/bf00580849
Abstract
Small columns packed with octadecasilylsilica were used to extract arginine-vasopressin in femtomole amounts from biological fluids for radioimmunoassay. This method is effective in isolating the peptide from substances in plasma and serum which interfere with its quantitation. Consistent and reproducible results and recoveries >80% were obtained with this procedure. High pressure liquid chromatography and serial dilution of extracted samples confirm the identity of the extracted product.Keywords
This publication has 14 references indexed in Scilit:
- Hydrophobic high-performance liquid chromatography of hormonal polypeptides and proteins on alkylsilane bonded silicaJournal of Chromatography A, 1979
- EXTRACTION OF SMALL AMOUNTS OF OXYTOCIN FROM BIOLOGICAL FLUIDS BY MEANS OF AGAROSE-BOUND NEUROPHYSINJournal of Endocrinology, 1979
- Characterization of an Antiserum Used in a Radioimmunoassay for Arginine-Vasopressin: Implications for Reference Standards*Endocrinology, 1978
- A NOVEL EXTRACTION METHOD FOR PLASMA VASOPRESSIN AND ITS APPLICATION IN A RADIOIMMUNOASSAYJournal of Endocrinology, 1978
- A Highly Specific Antiserum for Arginine Vasopressin1Endocrinology, 1977
- Radioimmunoassay Measurement of Arginine Vasopressin in Serum: Development and ApplicationJournal of Clinical Endocrinology & Metabolism, 1974
- Development and Clinical Application of a New Method for the Radioimmunoassay of Arginine Vasopressin in Human PlasmaJournal of Clinical Investigation, 1973
- Radioimmunoassay of posterior pituitary peptides: A reviewMetabolism, 1973
- RADIOIMMUNOASSAY FOR PLASMA ANTIDIURETIC HORMONEJournal of Endocrinology, 1972
- IMMUNOASSAY OF PLASMA VASOPRESSIN IN MANProceedings of the National Academy of Sciences, 1970