Generation of non MHC restricted killing in non‐cytotoxic T‐lymphocytes from leukaemia patients

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Abstract
Summary.Experiments were undertaken to determine whether the depression of natural killer (NK) activity previously observed in the peripheral blood lymphocytes (PBL) of leukaemia patients in remission extended to NK‐like precursors in the blood. T‐lymphocytes (E +) from leukaemia patients and normal subjects were depleted of IgG Fc‐receptor‐bearing (YFcR) fresh NK cells by passage over immune complex‐coated monolayers.yFcR —E + PBL were cultured alone or with DAUDI cells. On day 5 of culture, cytotoxicity toward the NK‐sensitive cell lines K562 and MOLT‐4 was evaluated in the responder lymphocytes of leukaemia patients and controls. Negligible NK‐like cytotoxicity was found in both FcR —E +PBI, responder populations cultured alone. By contrast, stimulation with DAUDI induced high levels of K562 and MOLT‐4 cytotoxicity in leukaemia as well as in normal responder cells. Complement‐mediated cytotoxicity experiments using various McAb demonstrated that in both normal and leukaemia cultures NK‐like effectors react with the pan‐T OKT3 McAb and with the OKTl1 McAR directed to the SRBC receptor, but not with Leullb and OKMl McAbs, directed against antigens expressed on peripheral blood NK cells. Fractionation of the responder cells on discontinuous Percoll gradients showed that most of this activity was present in the highly dividing blast cell fraction.

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