Induction of two genes by glucose starvation in hamster fibroblasts.

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Abstract
The coordinated expression fo 2 genes specificially induced by glucose starvation is demonstrated in a hamster fibroblast cell line, K12. Using 2 c[complementary]DNA plasmids , p4A3 and p3C5, as hybridization probes, the kinetics of induction of these genes were examined when the cells were grown in medium deprived of glucose. After a lag period of .apprx. 8 h, there is a rapid and simultaneous increase of the p4A3 and p3C5 mRNA levels, and the elevation of the mRNA levels for p4A3 and p3C5 is largely due to new transcription. The mRNA transcripts encoded by these glucose-regulated genes in culture cells and phosphoenolpyruvate carboxykinase, the enzyme that catalyzes the rate-limiting step in gluconeogenesis in fasted rats were compared. Evidently, the expression of phosphoenolpyruvate carboxykinase is not inducible by glucose starvation in the culture cells.

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