A Method for Isolating Continuous Cell Lines from Toxorhynchites Amboinensis (Diptera: Culicidae)1
- 30 April 1981
- journal article
- research article
- Published by Oxford University Press (OUP) in Journal of Medical Entomology
- Vol. 18 (2) , 140-144
- https://doi.org/10.1093/jmedent/18.2.140
Abstract
Minced larval tissue fragments and mechanically damaged but living whole larvae were compared as sources of seed cells for initiating continuous cell lines from Toxorhynchitesamboinensis.No cell line was obtained from the completely minced larval tissue fragments, although patches of cells that attached to the glass often replicated for 3 to 8 weeks. On the other hand, more than 20 continuous cell lines were isolated from the mechanically damaged but living larvae. The presence of 5 or more damaged larvae in tubes containing glass-attached cells during the 1st 8 weeks in primary culture was found to be important for isolating continuous cell lines from the mosquito. Cell lines were isolated faster when more damaged larvae were present in a tube.This publication has 1 reference indexed in Scilit:
- A Method for the Isolation and Identification of Dengue Viruses, using Mosquito Cell Cultures *The American Journal of Tropical Medicine and Hygiene, 1979