Import of precursor proteins into Vicia faba mitochondria

Open Access
Publisher Website
Google Scholar
Abstract
The ADP/ATP translocator from Vicia faba and β F1‐ATPase from Neurospora crassa were imported into plant mitochondria from a wheat germ and a rabbit reticulocyte translation system. Incubation of Vicia mitochondria in postribosomal supernatants of the translation reactions resulted in import of the ADP/ATP translocator. Precursor to the β‐subunit of the F1‐ATPase from N. crassa was imported and processed by the Vicia mitochondria. Import into a protease‐protected location was abolished by the inclusion of apyrase or valinomycin in the import mixtures. The Neurospora β‐subunit precursor showed post‐maturation processing to a protein with a molecular mass equivalent to that of the plant β‐subunit.