Promoter-regulatory region of the major immediate early gene of human cytomegalovirus.
- 1 February 1984
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 81 (3) , 659-663
- https://doi.org/10.1073/pnas.81.3.659
Abstract
The DNA templates containing immediate early (IE) genes of human cytomegalovirus (CMV) were transcribed in vitro by using a human cervical carcinoma HeLa cell extract. When IE region 1, 2 and 3 were used, transcription was detected qualitatively only from IE region 1. Transcription was detected with DNA representing IE region 2 when the IE region 1 promoter was not present. DNA sequence analysis of the upstream regulatory region of IE region 1 detected 2 distinct repeats of 19 and 18 nucleotides, both being repeated 4 times. A putative cruciform structure could form through the surrounding sequences with each 18-nucleotide repeat being located in the unpaired region. The potential secondary structure and the repeat sequences in the regulatory region of IE region 1 are presumably related to the high level of transcription of the IE gene.This publication has 38 references indexed in Scilit:
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