Different thermal energy requirement for open complex formation byEscherichia coliRNA polymerase at two related promoters
Open Access
- 25 November 1991
- journal article
- research article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 19 (22) , 6113-6118
- https://doi.org/10.1093/nar/19.22.6113
Abstract
We have studied the effect of temperature on transcription initiation in vitro at two related promoters galPcon and galP1, which have the same nucleotide sequence around the −10 region and transcription start site, but differ in upstream sequences. One of the promoters, galPcon, carries the consensus -35 hexamer, 5′TTGACA 3′, whilst galP1 contains a block of ‘distortable’ upstream sequences that allow promoter function in the absence of a −3 5 region consensus sequence. RNA polymerase can form complexes with both promoters at a range of temperatures. However, the thermal energy requirement for open complex formation differs: open complexes can form at galP1 at low temperatures, whereas galPcon requires higher temperatures. The thermal energy requirement for transcription from preformed open complexes is the same for both promoters.Keywords
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