Estimation of (capon) comb growth hormone in urine

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Abstract
When urine, after addition of 150 ml. 25% HCl per liter, is heated for 2 periods of 6 hr. in presence of benzene no loss of comb growth-promoting substances occurs. If 10 ml. 25% HCl are added instead of 150 ml., the yield of comb growth-promoting hormones remains the same. Free androsterone and/or dehydroandrosterone added to urine freed from comb growth-promoting substances beforehand and extracted with benzene in the presence of 150 ml. 25% HCl per 1. on a boiling water bath for 2 periods of 6 hrs. are not destroyed by this method. When these acidified urines containing added free hormones are boiled without benzene a loss of 60% of activity occurs in 15 mins. and more than 80% in 2 hrs. Urine boiled with 1/10 vol. 25% HCl in absence of benzene for 15 min. may lose none of its [male] hormone activity; when boiled for 2 hrs., some loss occurs. Boiling urine with double the quantity of acid always diminishes the content of comb growth-promoting substance. After boiling urine in absence of benzene for 2 hrs. with 1/10 vol. 25% HCl, no dehydroandrosterone could be detected.

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