The common 5′ terminal sequence on trypanosome mRNAs: a target for anti-messenger oligodeoxynucleotides
Open Access
- 11 July 1986
- journal article
- research article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 14 (14) , 5605-5614
- https://doi.org/10.1093/nar/14.14.5605
Abstract
Several mature mRNAs of Trypanosoma brucel were previously shown to have a common 5′ terminal sequence of 35 nucleotldes (nt) encoded by a separate mini-exon. To verify whether all trypanosome mRNAs contain this mini-exon sequence at their 5′ end, we have tested oligodeoxynucleotides complementary to different parts of the 35 nt leader sequence for their ability to inhibit translation of total trypanosome mRNA. All ollgomers tested inhibited translation of trypanosome mRNAs in a wheat germ extract. They had no effect on translation of Brome mosaic virus mRNA and of a trypanosome mRNA for phos-phoglycerate kinase modified to remove the mini-exon sequence. Three different 12mers inhibited translation 35–60%; both the 22- and 34mer inhibited translation 95–100%. Incorporation of amino acids decreased proportionally in all protein bands detected in high resolution polyacrylamide gels. Our results show that all trypanosome mRNAs that yield a product detectable in gel contain a mini-exon sequence. He infer that most, if not all, trypanosome mRNAs contain a 5′ terminal mini-exon sequence ac′ quired by discontinuous synthesis.Keywords
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