Differential Cytotoxicity of Activated Lymphocytes on Allogeneic and Xenogeneic Target Cells
- 1 May 1976
- journal article
- research article
- Published by Wiley in Scandinavian Journal of Immunology
- Vol. 5 (5) , 479-486
- https://doi.org/10.1111/j.1365-3083.1976.tb00302.x
Abstract
A competitive inhibition assay was used to define the specificity of the in vitro cytotoxicity of activated lymphocytes. Human and mouse effector cells were produced by prestimulation with phytohemagglutinin, pokeweed mitogen or purified protein derivative for several days. The addition of increasing numbers of unlabeled [human liver] Chang cells to a fixed number of stimulated human lymphocytes and 51Cr-labeled Chang cells gradually decreased Cr release. Admixture of unlabeled human bladder tumor [T24 and RT4] cells, lung cells or [green] monkey kidney [Vero] cells had a similar effect, whereas mouse [fibroblast] L cells were not inhibitory. The reverse was true when stimulated mouse lymphocytes and 51Cr-labeled mouse L cells were incubated. In this case, the addition of unlabeled L or rat [bladder] tumor [Bcl] cells effectively inhibited 51Cr release, whereas human or monkey cells were less inhibitory. Human and mouse cells inhibited the cytotoxicity of human effector lymphocytes towards mouse L cells. The cytotoxic effects of mouse lymphocytes on human cells were also decreased by addition of unlabeled human, monkey or mouse cells, but in this case human cells were most inhibitory. Activated lymphocytes apparently recognize several surface structures on the target cells. Some of these structures may be shared by cells of different species origin, whereas others seem to be unique for cells from phylogenetically closely related species.This publication has 6 references indexed in Scilit:
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