In vivo phosphorylation of the 30‐kDa protein of tobacco mosaic virus

Abstract
The 30‐kDa protein of tobacco mosaic virus, which is involved in cell‐to‐cell movement function, is phosphorylated in tobacco protoplasts. To investigate which portion of the protein is phosphorylated we inoculated several truncated 30‐kDa protein mutants into protoplasts and determined whether or not those truncated proteins are phosphorylated. The results showed that amino acid residues 234–261 of the 30‐kDa protein are required for this phosphorylation.