Autophosphorylation of Yeast Hexokinase PII
- 1 September 1988
- journal article
- research article
- Published by Microbiology Society in Microbiology
- Vol. 134 (9) , 2493-2498
- https://doi.org/10.1099/00221287-134-9-2493
Abstract
Autophosorylation of hexokinase PII was studied using an enzyme purified from Saccharomyces cerevisiae. Incubation of this enzyme preparation with [.gamma.-32P]ATP and Mn2+ or Mg2+ gave a phosphoprotein of molecular mass 58 000 which corresponded to hexokinase PII. D-Xylose stimulated autophosphorylation of hexokinase PII. Dilution of hexokinase PII over a 10-fold concentration range did not change the specific activity of hexokinase PII autophosphorylation, suggesting that it may occur by an intramolecular mechanism.This publication has 13 references indexed in Scilit:
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