THE HETEROMORPHIC MARKER ON CHROMOSOME-18 USING RESTRICTION ENDONUCLEASE ALUI
- 1 April 1986
- journal article
- research article
- Vol. 38 (4) , 549-554
Abstract
The staining property of pericentromeric heterochromatin of chromosome 18 is compared by C-banding and restriction endonuclease AluI digestion methods. Only a small distal fraction of C-band of chromosome 18 is observed to be resistant to AluI treatment, which positively stained with subsequent Giemsa staining. The resitant fraction is characteristic and usually located toward the short arm. The extensive heterogeneity of constitutive heterochromatin revealed by AluI treatment is useful in demonstrating the heterozygosity of homologous chromosomes. This, in turn, may provide frequent markers to identify the chromosomes 18''s. This present approach can be utilized in evaluation of the families to describe the origin of the extra chromosome 18 in Edward syndrome. As an example, one such family has been investigated where the additional chromosome 18 originated due to paternal nondisjunction at meiosis I.This publication has 13 references indexed in Scilit:
- TRISOMY IN MANAnnual Review of Genetics, 1984
- The frequency of 47,+21, 47,+18, and 47,+13 at the uppermost extremes of maternal ages: results on 56,094 fetuses studied prenatally and comparisons with data on livebirthsHuman Genetics, 1984
- Origin of nondisjunction in trisomy 21 syndrome: All studies compiled, parental age analysis, and international comparisonsAmerican Journal of Medical Genetics, 1983
- Rates of trisomies 21, 18, 13 and other chromosome abnormalities in about 20 000 prenatal studies compared with estimated rates in live birthsHuman Genetics, 1982
- Parental origin of chromosomes in Down's syndromeHuman Genetics, 1981
- Association of ribosomal genes in the fibrillar center of the nucleolus: a factor influencing translocation and nondisjunction in the human meiotic oocyte.Proceedings of the National Academy of Sciences, 1980
- Human Chromosomal Heteromorphisms: Nature and Clinical SignificancePublished by Elsevier ,1980
- A simple technique for demonstrating centromeric heterochromatinExperimental Cell Research, 1972