Preparation and Characterization of Actin from Liver
Open Access
- 1 October 1983
- journal article
- Published by Walter de Gruyter GmbH in Zeitschrift für Naturforschung C
- Vol. 38 (9-10) , 829-833
- https://doi.org/10.1515/znc-1983-9-1025
Abstract
G-actin was prepared from pork liver by gelfiltration on AcA 44 Ultrogel. The purity of the actin was 95-98% and the yield was 45-150 mg from 500 g liver, corresponding to a maximum efficiency of recovery of about 30%. High viscosities of actin-enriched solutions were reduced by a specific pH-step. In SDS-gel electrophoresis a single band of about ~ 45000 daltons was seen. The G-actin was polymerizable as shown by high shear viscosity measurements. The isoelectric points of the β- and γ-isoactins were 5.6 and 5.7.Keywords
This publication has 0 references indexed in Scilit: