Effect of Synthetic Lysine Polypeptides on Rabbit Blood Coagulation.

Abstract
Synthetic polypeptides were investigated for their effects on rabbit blood coagulation in vitro and in vivo. The basic L-lysine polypeptides prolonged whole-blood clotting time, increased prothrombin time and neutralized the effect of heparin. A synthetic L-glutamic acid polypeptide inhibited these effects of polylysine and protamine. In mice, guinea pigs, and rabbits the lysine polypeptides produced anaphylactoid symptoms which closely resembled those of protamine toxicity. A mechanism involving a combination of the basic polypeptides with acidic protein surfaces or with heparin is discussed to explain some of these re suits.

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