NMR spectroscopy of human post mortem cerebrospinal fluid: Distinction of Alzheimer's disease from control using pattern recognition and statistics

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Abstract
1H NMR spectra have been measured at 500 and 600 MHz on 23 human cerebrospinal fluid samples obtained at autopsy from Alzheimer's disease patients and controls. The spectra at 500 MHz were quantified using 42 descriptors based on NMR peak heights and it was shown that differences between the two classes were apparent in the δ2.4–2.9 region. Remeasured at 600 MHz a detailed examination of this chemical shift range identified citrate, aspartate, N‐acetyl aspartate, methionine and glutamate in this region of the spectra. Principal components analysis showed that a separation of the two classes was possible and detailed statistics indicated that citrate level was the principal marker. Patient age and the interval between death and autopsy (parameters not closely matched between the two groups) were examined statistically to establish whether these might account for the citrate differences. Although they could possibly account for them to some extent, the relationship between citrate levels and disease state remained significant at pex vivo.