CELLULAR CYTO-TOXICITY AGAINST CANINE ENDOTHELIAL-CELLS - ANALYSIS OF DETERMINANTS RECOGNIZED BY CTL

  • 1 January 1983
    • journal article
    • research article
    • Vol. 21  (2) , 114-128
Abstract
A method is described which permits culture of both arterial and venous canine endothelial cells. Cell-mediated cytotoxicity against cultured endothelial cells was studied. A 51Cr-release assay was used to detect CTL [cytotoxic T lymphocytes] generated in MLC [mixed leukocyte culture]. Both arterial and venous endothelial cells are lysed by CTL specifically. Cold target inhibition experiments were performed to analyze the CTL-recognized antigens on arterial and venous endothelial cells. Different antigens are recognized by CTL on venous endothelial cells and PHA[phytohemagglutinin]-blasts. CTL possibly recognize venous endothelial cells through class II antigens or E-M antigens. Arterial endothelial cells and PHA-blasts share CTL-recognized antigens.

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