Purification and characterization of two new modification methylases; MClal from Caryophanon latum L and MTaqI from Thermus aquaticus YTI

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Abstract
A method for detecting Type II modification methylases and determining their methylation site by assaying the ability of methylated DNA to be cleaved by heterologous restriction enzymes is described and applied to the isolation of the restriction modification methylases from Thermus thermophilus HB8, Thermus aquaticus YTI and Caryophanon latum L. M.TaqI is shown to have a methylation specificity identical to M.TthI (TCG me A). M.ClaI methylates at adenine and protects a subset of TthI sites indicating that it methylates the sequence ATCG me AT. Methylation by M.TthI also protects against cleavage by SalI, XhoI and at some HindII, AccI and MboI sites.