IN VITROSTUDIES ON STAPHYLOCOCCAL ENTEROTOXIN PRODUCTION

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Abstract
Quantitative difference in entero-toxin production by different subcultures of a single strain of Staphylo-coccus has been demonstrated. The size of the zone of enterotoxin-antienterotoxin precipitate around the colonies developing an antiserum agar could be used as a guide to the selection of inoculums for the best production of enterotoxin. The size of the precipitate halo is determined by the proportion in the colony of cells which produce and those that do not produce enterotoxin. A simplified method for the direct demonstration of the antigenic type of enterotoxin is described.