Staining Properties of Oil Red O and a Method of Partial Purification of the Commercial Product

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Abstract
1. A yellow-brown component of commercial oil red O separated by our technic of paper chromatography is shown to stain human serum proteins, particularly those that are coagulated by heat. The patterns of serum electropherograms obtained by coloring with the crude oil red O solution consist of a red lipid pattern superimposed on a brownish protein pattern. 2. A method is described for partial removal of the nonred components from the crude oil red O. Satisfactory coloration of lipoproteins was obtained with the "purified" oil red O. 3. It is demonstrated that areas of paper strip covered with protein may stain lighter with some oil red O fractions than the background filter paper.