Electrophoretic Identification of Genetic Markers in Channel Catfish and Blue Catfish by Use of Low-Risk Tissues
- 1 January 1992
- journal article
- Published by Oxford University Press (OUP) in Transactions of the American Fisheries Society
- Vol. 121 (1) , 26-35
- https://doi.org/10.1577/1548-8659(1992)121<0026:eiogmi>2.3.co;2
Abstract
Electrophoretic analysis of enzymes and proteins enables aquaculturists to distinguish among stocks of channel catfish Ictalurus punctatus and blue catfish I. furcatus, and to monitor gene flow, hybridization, and inbreeding. Sixteen previously unreported genetic markers allow much more precise resolution of stock differences. Analysis of low-risk tissue samples (caudal fin, adipose fin, and barbels) enables investigators to obtain genetic data with little stress on individual fish. We have identified at least 69 enzyme locus products in populations of channel catfish (Kansas, Marion, “Auburn,” Mississippi, and Red River strains) and blue catfish by use of autopsied tissues. Polymorphic loci account for at least 36% of the loci examined. Allelic polymorphisms fixed between blue and channel catfish were observed at 13 loci. Genetic variation was observed at 17 loci among channel catfish populations and at 5 loci in the single blue catfish population. Analysis of species-specific genetic markers am...Keywords
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