Tightly bound nucleotides affect phosphate binding to mitochondrial F1‐ATPase
- 25 March 1985
- journal article
- Published by Wiley in FEBS Letters
- Vol. 182 (2) , 425-428
- https://doi.org/10.1016/0014-5793(85)80347-1
Abstract
The interaction of inorganic phosphate with native and nucleotide‐depleted F1‐ATPase was studied. F1‐ATPase depleted of tightly bound nucleotides loses the ability to bind inorganic phosphate. The addition of ATP, ADP, GTP and GDP but not AMP, restores the phosphate binding. The nucleotides affecting the phosphate binding to F1‐ATPase are located at the catalytic (exchangeable) site of the enzyme. The phosphate is thought to bind to the same catalytic site where the nucleotide is already bound. It is thought that ADP is the first substrate to bind to F1‐ATPase in the ATP synthesis reaction.Keywords
This publication has 22 references indexed in Scilit:
- Recent developments on structural and functional aspects of the F1 sector of H+-linked ATPasesMolecular and Cellular Biochemistry, 1984
- Localization of the tight ADP‐binding site on the membrane‐bound chloroplast coupling factor oneEuropean Journal of Biochemistry, 1983
- The proton-ATPase of bacteria and mitochondriaThe Journal of Membrane Biology, 1983
- Chapter 16 An H+-ATPSynthetase: A Substrate Translocation ConceptPublished by Elsevier ,1982
- Correlation between the kinetics of activation and inhibition of adenosinetriphosphatase activity by divalent metal ions and the binding of manganese to chloroplast coupling factor 1Biochemistry, 1981
- The number and localisation of adenine nucleotide-binding sites in beef-heart mitochondrial ATPase (F1) determined by photolabelling with 8-azido-ATP and 8-azido-ADPBiochimica et Biophysica Acta (BBA) - Bioenergetics, 1980
- 4-Azido-2-nitrophenyl phosphate, a new photoaffinity derivative of inorganic phosphate. Study of its interaction with the inorganic phosphate binding site of beef heart mitochondrial adenosine triphosphataseBiochemistry, 1980
- Mg2+-Induced ADP-dependent inhibition of the ATPase activity of beef heart mitochondrial coupling factor F1Biochemical and Biophysical Research Communications, 1979
- The interactions of coupling ATPases with nucleotidesBiochimica et Biophysica Acta (BBA) - Reviews on Bioenergetics, 1978
- The structure of mitochondrial ATPaseBiochimica et Biophysica Acta (BBA) - Reviews on Bioenergetics, 1973