Correlation of In Vivo Malignancy With In Vitro Properties of Human-Mouse Hybrid Cells 2

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Abstract
The in vivo tumorigenicity of malignant mousenonmalignant human somatic cell hybrids was correlated with the in vitro characteristics. The renal adenocarcinoma mouse cell line RAG and the normal, diploid human cell line WI-38 were used as the fusion parents. Five independent RAG-WI-38 hybrid clones were tested for concanavalin A (Con A) agglutination patterns, in vitro invasiveness, and tumor formation in immunosuppressed mice. Tests on the parental lines showed that RAG cells agglutinated at much lower levels of Con A than the WI-38 cells. RAG cells overgrew WI-38 cells in the in vitro invasiveness assay. RAG cells readily formed tumors in untreated adult or young immunosuppressed mice, whereas the WI-38 cells did not. The five hybrid clones were all similar. since they had Con A agglutination levels intermediate to those of both parents, though patterns were more “tumor-like”, overgrew the WI-38 cells in the invasiveness assay, and formed tumors in immunosuppressed...

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