Blood Group Antigens in Butanol Extracts of Animal Tissues

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Abstract
Aqueous phase fractions obtained after extraction with n-butanol of erythrocytes and organ tissues of rabbits, guinea pigs and monkeys were examined for H, HI, I and i blood group activity in inhibition of agglutination experiments using red cells and anti-H, anti-HI, anti-I and anti-i sera of human origin. Rabbit and guinea pig extracts of erythrocytes and some but not all organ tissues studied had H, HI and I but no i blood group activity. Monkeys had i substance only in the extracts of red cells and H substance only in the extracts of gastric mucosa. The I and HI substances were not demonstrated in any of the extracts of monkey tissues or red cells.