Abstract
T. acidophila, a mycoplasma-like organism, was grown at 56.degree. C and pH 2. The microfluidity of membrane vesicles was determined for the temperature range -2.degree.-35.degree. C using spin labels. The temperature at which the low-temperature phase transition occurred in membrane lipids increased with increasing Ca2+ concentration. There was no evidence of a low-temperature lipid phase transition for membrane vesicles suspended in a medium containing monovalent instead of bivalent cations.

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