Black Raspberry Extract and Fractions Contain Angiogenesis Inhibitors
- 19 April 2005
- journal article
- research article
- Published by American Chemical Society (ACS) in Journal of Agricultural and Food Chemistry
- Vol. 53 (10) , 3909-3915
- https://doi.org/10.1021/jf048585u
Abstract
Targeted therapies, such as agents that inhibit angiogenesis, offer hope as complementary agents in cancer therapy. Angiogenesis-inhibiting agents have the potential for inhibiting tumor growth and limiting the dissemination of metastasis, thus keeping cancers in a static growth state for prolonged periods. Black raspberry (Rubus occidentalis) extract was discovered to be antiangiogenic (0.1% w/v) in a novel human tissue-based in vitro fibrin clot angiogenesis assay. Assay-guided fractionation of a crude black raspberry extract resulted in a highly potent antiangiogenic fraction that accounted for only 1% of the fresh weight of whole black raspberries. At 0.075% (w/v), the active fraction completely inhibited angiogenic initiation and angiogenic vessel growth. Further subfractionation of this active fraction revealed the coexistence of multiple antiangiogenic compounds, one of which has been identified as gallic acid. However, the individual subfractions did not outperform the active whole fraction. These findings suggest that an active black raspberry fraction may be a promising complementary cancer therapy. It is natural and potent enough for manageable dosing regimens. These extracts contain multiple active ingredients that may be additive or synergistic in their antiangiogenic effects. These observations warrant further investigations in animals and human trials. Keywords: Angiogenesis; black raspberries; extraction; fingerprinting; fractionation; initiation; human tissue; neovessels; standardization; synergismKeywords
This publication has 16 references indexed in Scilit:
- Cancer without diseaseNature, 2004
- Anti-angiogenic, Antioxidant, and Anti-carcinogenic Properties of a Novel Anthocyanin-Rich Berry Extract FormulaBiochemistry (Moscow), 2004
- Development of Targeted Somatostatin-Based Antiangiogenic Therapy: A Review and Future PerspectivesCancer Biotherapy & Radiopharmaceuticals, 2003
- Anthocyanin Glycosides from Berry Fruit Are Absorbed and Excreted Unmetabolized by Both Humans and RatsJournal of Agricultural and Food Chemistry, 2003
- Angiogenesis in health and diseaseNature Medicine, 2003
- Inhibition of angiogenic initiation and disruption of newly established human vascular networks by juice from Morinda citrifolia (noni)Angiogenesis, 2003
- Cyclooxygenase inhibitory and antioxidant cyanidin glycosides in cherries and berriesPhytomedicine, 2001
- Patterns and Emerging Mechanisms of the Angiogenic Switch during TumorigenesisPublished by Elsevier ,1996
- Choroidal Neovascularization Associated with Choroidal NeviAmerican Journal of Ophthalmology, 1978
- Anti-AngiogenesisAnnals of Surgery, 1972