Alfatoxin B1-DNA adducts and hepatitis B virus antigens in hepatocellular carcinoma and non-tumorous liver tissue

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Abstract
Studies were carried out to test the hypothesis that exposure to aflation B1 (AFB1) is comman among individuals with hepatocellular carcinoma (HCC) who are also chronically infected with hapititis B virus (HBV). Experiments were also carried out to determine whether there is a close association between the presence of AFB1-DNA adducts and the expression of one or more HBV antigens in the tumor or non-tumor regions of the liver. Twently-seven paired tumor and non-tumor liver tissues of HCC patients from Taiwan were analyzed. Monoclonal antibody 6A10, generated against the imidazole ring-opened persistent form of the major N-7 guanine adduct of AFB1, was used for adduct detection by both indirect immunofluorescence and competitive enzymelinked immunosorbent assay. An avidin-biotin complex staining method was used for the detection of HBsAg and HBxAg in Liver sections. A total of 8 (30%) HCC samples and 7 (26%) adjacent non-tumor liver tissue samples from Taiwan were positive for AFB1-DNA adducts. For HBsAg, 10 (37%) HCC samples and 22 (81%) adjacent non-tumorous liver samples were positive while 9 (33%) HCC samples and 11 (41%) adjacent non-tumor liver samples were HBxAgpositive. No association with AFB1-DNA adducts was observed for HBsAg and HBxAg. These results suggest that both AFB, exposure and carrier status of HBsAg/HBxAg may be involved in the induction of HCC in Taiwan.

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