Scale: a chemical approach for fluorescence imaging and reconstruction of transparent mouse brain
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- 30 August 2011
- journal article
- Published by Springer Nature in Nature Neuroscience
- Vol. 14 (11) , 1481-1488
- https://doi.org/10.1038/nn.2928
Abstract
The authors describe a chemical approach for imaging deep into fixed brain tissue using Scale, a solution that renders biological samples transparent, but preserves fluorescent signals. This technique allows for imaging at unprecedented depth and at subcellular resolution, and makes three-dimensional reconstruction of neural networks possible without serial sectioning.Keywords
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