Blastocyst Axis Is Specified Independently of Early Cell Lineage But Aligns with the ZP Shape
- 4 May 2007
- journal article
- research article
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 316 (5825) , 719-723
- https://doi.org/10.1126/science.1138591
Abstract
The mechanisms controlling the establishment of the embryonic-abembryonic (E-Ab) axis of the mammalian blastocyst are controversial. We used in vitro time-lapse imaging and in vivo lineage labeling to provide evidence that the E-Ab axis of the mouse blastocyst is generated independently of early cell lineage. Rather, both the boundary between two-cell blastomeres and the E-Ab axis of the blastocyst align relative to the ellipsoidal shape of the zona pellucida (ZP), an extraembryonic structure. Lack of correlation between cell lineage and the E-Ab axis can be explained by the rotation of the embryo within the ZP.Keywords
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