Notizen: Über ein zellfreies System der Proteinsynthese aus Chlorella
Open Access
- 1 October 1966
- journal article
- Published by Walter de Gruyter GmbH in Zeitschrift für Naturforschung B
- Vol. 21 (10) , 993-995
- https://doi.org/10.1515/znb-1966-1017
Abstract
From cell homogenates of Chlorella pyrenoidosa, a system has been prepared which incorporates amino acids into acid-insoluble peptides. This system is dependent on energy regeneration (with phosphoenolpyruvate and pyruvate kinase), on GTP, and, to a lesser extent on CTP and UTP. Experiments with washed ribosomes show, a linear dependency of incorporation on ribosome concentration. By using a "pH-5-enzyme” preparation instead of the supernatant fraction, the incorporation can be stimulated by adding nucleic acids.Keywords
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